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Naik, Veena V.
- Differentiation of Bone and Soft Tissue Using Methylene Blue - Acid Fuchsin: a New Stain Combination
Authors
1 Department of Oral Pathology, KLE VK Institute of Dental Sciences, KLE University, Nehru Nagar, Belgaum-590010, Karnataka, IN
2 Department of Oral Pathology, Bharatiya Vidhyapeeth Dental College, Sangli, Maharashtra, IN
3 Dept. of Pathology, JN Medical College, Belgaum-10, IN
4 Department of Oral Pathology, ITS Dental College & Hospital, Noida, IN
Source
Indian Journal of Public Health Research & Development, Vol 3, No 1 (2012), Pagination: 182-184Abstract
Introduction
Hematoxylin-Eosin(H-E) provides optimal staining for histology, but in formalin-fixed, decalcified, paraffinembedded bone tissue, both bone and soft tissue appear the same shade of pink. In addition to the improved staining properties, the methylene blue-acid fuchsin stain is also superior to H-E in terms of its stability, cost and ease of use.
Aim & Objectives
To determine the efficacy of methylene blue-acid fuchsin stain combination to differentiate mineralized structures from surrounding soft tissues in various oral lesions.
Material & Methods
Two sections each of peripheral cemento-ossifying fibroma, central cemento-ossifying fibroma, fibroma with calcification, fibrous dysplasia, adenomatoid odontogenic tumour and pleomorphic adenoma were taken. One section was stained with hematoxylin-eosin and the other with methylene blue-acid fuchsin.
Results & Conclusion
In contrast to standard hematoxylin-eosin stain, which stains both bone and soft tissues pink, the methylene blue-acid fuchsin stain combination demonstrates remarkable contrast with bone appearing bright pink and the soft tissue blue-purple.
Keywords
Methylene Blue, Acid Fuchsin, Bone, Soft Tissue
References
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- Expression of Perlecan (Heparan Sulphate Proteoglycan) in Oral Squamous Cell Carcinoma
Authors
1 Department of Oral Pathology, KLE VK Institute of Dental Sciences, Belgaum, IN
2 Department of Oral Pathology, ITS Dental College & Hospital, Noida, IN
3 Department of Pathology, JN Medical College, Belgaum, IN
4 Department of Public Health Dentistry, KLE VK Institute of Dental Sciences, Belgaum, IN
Source
Indian Journal of Public Health Research & Development, Vol 3, No 1 (2012), Pagination: 185-188Abstract
Background
Perlecan is a basement membrane heparan sulfate proteoglycan. It plays a vital role in cell- cell adhesion, cell-matrix adhesion and is associated with several growth factors. Recently its role has been found in many pathological and physiological conditions. Aim of this study was to understand the immunolocalisation of perlecan in normal mucosa and oral squamous cell carcinoma and also to derive a correlation between perlecan expression and various grades of carcinoma.
Method
A total of thirty tissue blocks comprising of ten normal mucosa and twenty oral squamous cell carcinoma were included in the study. They were examined for the presence of perlecan protein core by immunohistochemistry using monoclonal antibody (Anti-Basement Membrane-type heparan sulfate proteoglycan core protein. Specificity: Mouse monoclonal anti human Perlecan Clone No: 85-9). Interpretation of staining was done and the observations statisticaly analysed by Fisher exact tests to evaluate the expression of perlecan within and around the tumor islands.
Results
In normal epithelium perlecan expression was limited to basal layer. In oral squamous cell carcinoma, perlecan was present in surface epithelium, stroma as well as tumor islands. Perlecan expression within tumor islands became scarce with the higher grades of carcinoma.
Conclusions
It was deduced from the results that with the increase in degree of carcinoma; more heparanase enzyme acts on perlecan and from the breakdown of perlecan; fibroblast growth factor, transforming growth factor-β and other growth factors are released. All these factors are known to promote tumor growth.
Keywords
Perlecan, Immunohistochemistry, Heparan Sulphate Proteoglycan, Oral Cancer, Growth Factors, Squamous Cell CarcinomaReferences
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- Comparison and Correlation of Glucose Levels in Serum and Saliva of Patients with Diabetes Mellitus
Authors
1 Department of Oral Pathology, KLE VK Institute of Dental Sciences, KLE University, Belgaum, IN
2 Department of Oral Pathology, Goa Dental College & Hospital, Goa, IN
3 Department of Pathology, JN Medical College, KLE University, Belgaum, IN
4 Department of Oral Pathology, ITS Dental College & Hospital, Noida, IN
Source
Indian Journal of Public Health Research & Development, Vol 2, No 1 (2011), Pagination: 103-105Abstract
Background and objectives
In Diabetes Mellitus, an important aspect of glycaemic control is to regularly monitor glucose levels. Current methods employed, either require a blood sample or urine sample. These procedures usually cause pain and discomfort to the patient. Hence, arises the need for a noninvasive technique in diagnosis and in monitoring glycaemic status of an individual. The study was undertaken in an attempt to compare and correlate glucose levels in saliva and serum of patients with diabetes and non-diabetic healthy individuals, to determine the efficacy of saliva as a diagnostic aid.
Method
250 individuals visiting diabetic clinics were screened randomly. Of these, 200 were confirmed diabetics and were under medication (Study Group). The remaining 50 gave neither a past history of diabetes nor did their present glycemic status depicted high values (Control Group). Venous blood and salivary samples were obtained from each individual and subjected to glucose estimation. Both fasting and post-prandial samples were analyzed.
Results and observations
Glucose was detected in the saliva of both diabetic and non-diabetics. The fasting salivary glucose values in the control group ranged from 4.1 to 13.3 mg/dl and the postprandial salivary glucose values from 12.5 to 20.0 mg/dl. The fasting salivary glucose values in the study group ranged from 4.1 to 26.6 mg/dl and the Post-prandial salivary glucose values from 15.3 to 30.7 mg/dl. It was observed that as blood glucose levels changed in both fasting and post-prandial samples, so did salivary glucose levels, irrespective of age and sex. A significant P value < 0.001 and positive correlation was found between blood glucose and salivary glucose levels in both the diabetics and the controls.
Conclusion
It can thus be inferred that saliva can be used as an adjunct diagnostic tool in Diabetes Mellitus.
Keywords
Diabetes Mellitus, Glycaemic Status, Salivary Glucose, Non Invasive Diagnostic Tools in Diabetes, Saliva in DiabeticsReferences
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